Dewi, Ni Luh Putri Candra (2026) UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN PARIJATA (Medinilla Speciosa Blume) TERHADAP Streptococcus pyogenes DENGAN METODE DIFUSI CAKRAM. Diploma thesis, Poltekkes Kemenkes Denpasar Jurusan Teknologi Laboratorium Medis 2026.
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Abstract
ANTIBACTERIAL ACTIVITY TEST OF PARIJATA LEAF ETHANOL EXTRACT (Medinilla Speciosa Blume) AGAINST Streptococcus pyogenes WITH DISC DIFFUSION METHOD ABSTRACT Acute Respiratory Infections (ARI) remain a health problem in Indonesia, one of which is caused by Streptococcus pyogenes. Parijata leaves (Medinilla Speciosa Blume) contain secondary metabolites such as flavonoids, saponins, and tannins, which potentially have antibacterial activity. This study aimed to determine the antibacterial activity of parijata leaf ethanol extract against S. pyogenes using the disk diffusion method. This study was a true experimental study with a posttest only control design. The extract was obtained by maceration using 70% ethanol and tested at concentrations of 2%, 4%, 6%, 8%, and 10%. The results showed mean inhibition zone diameters of 7.27 mm, 8.06 mm, 8.95 mm, 9.37 mm, and 10.35 mm, respectively. Concentrations of 2%, 4%, 6%, and 8% were categorized as moderate, while 10% was categorized as strong. One-Way ANOVA showed a significant difference among concentrations with a Sig. value of 0.000 < 0.05. Therefore, parijata leaf ethanol extract has antibacterial activity against S. pyogenes and has potential to be developed as a natural antibacterial agent. Keywords: parijata leaf, antibacterial, Streptococcus pyogenes UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN PARIJATA (Medinilla Speciosa Blume) TERHADAP Streptococcus pyogenes DENGAN METODE DIFUSI CAKRAM ABSTRAK Infeksi Saluran Pernapasan Akut (ISPA) masih menjadi masalah kesehatan di Indonesia, salah satunya yang disebabkan oleh Streptococcus pyogenes. Daun parijata (Medinilla Speciosa Blume) mengandung senyawa metabolit sekunder, seperti flavonoid, saponin, dan tanin, yang berpotensi memiliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak etanol daun parijata terhadap pertumbuhan S. pyogenes dengan metode difusi cakram. Penelitian ini merupakan penelitian true experimental dengan rancangan posttest only control design. Ekstrak daun parijata diperoleh melalui metode maserasi menggunakan etanol 70% dan diuji pada konsentrasi 2%, 4%, 6%, 8%, dan 10%. Hasil penelitian menunjukkan rerata diameter zona hambat berturut-turut sebesar 7,27 mm; 8,06 mm; 8,95 mm; 9,37 mm; dan 10,35 mm. Konsentrasi 2%, 4%, 6%, dan 8% termasuk kategori sedang, sedangkan konsentrasi 10% termasuk kategori kuat. Hasil uji One-Way ANOVA menunjukkan adanya perbedaan yang bermakna antar konsentrasi dengan nilai Sig. 0,000 < 0,05. Dengan demikian, ekstrak etanol daun parijata memiliki aktivitas antibakteri terhadap S. pyogenes dan berpotensi dikembangkan sebagai antibakteri alami. Kata kunci: daun parijata, antibakteri, Streptococcus pyogenes
| Item Type: | Thesis (Diploma) |
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| Uncontrolled Keywords: | parijata leaf, antibacterial, Streptococcus pyogenes daun parijata, antibakteri, Streptococcus pyogenes |
| Subjects: | Q Science > Q Science (General) Q Science > QR Microbiology |
| Divisions: | Jurusan Analis Kesehatan |
| Depositing User: | Paramita Ida Ayu Nia |
| Date Deposited: | 13 Aug 2026 06:06 |
| Last Modified: | 13 Aug 2026 06:06 |
| URI: | http://repository.poltekkes-denpasar.ac.id/id/eprint/21060 |
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