Anjani, Ni Putu Lisa (2025) UJI AKTIVITAS ANTIBAKTERI STAPHYLOCOCCUS AUREUS BERDASARKAN PERBEDAAN JENIS PELARUT EKSTRAK BIJI BUAH PEPAYA (CARICA PAPAYA L.). Diploma thesis, Politeknik Kesehatan Denpasar Jurusan Teknologi Laboratorium Medis 2024.
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Abstract
ANTIBACTERIAL ACTIVITY TEST OF Staphylococcus aureus BASED ON DIFFERENT TYPES OF SOLVENTS FROM PAPAYA SEED EXTRACT (Carica papaya L.) ABSTRACT Staphylococcus aureus is an opportunistic pathogenic bacteria on the surface of the skin that causes impetigo. Treatment therapy for impetigo can be done by administering natural medication from papaya seeds (Carica papaya L). This study aims to determine the antibacterial activity of Staphylococcus aureus papaya seed extract based on the different types of extract solvents used. This research is true experimental research with a posttest only control design research form. The research method used was the disk diffusion method with three treatments of papaya seed extract, namely 96% ethanol extract, ethyl acetate and n-hexane, positive control using chloramphenicol and negative control using 96% ethanol, ethyl acetate and n-hexane. The research results showed that each papaya seed extract treatment was able to inhibit Staphylococcus aureus with an inhibition zone of 96% ethanol extract of 18.09 mm, ethyl acetate extract of 7.93 mm, n-hexane extract of 0.36 mm. The One Way Anova test showed a value of ρ<α(0.05) so that there was a significant difference in the diameter of the inhibition zone in each extract treatment. The Tukey test showed that there were significant differences in the inhibition zone of Staphylococcus aureus bacteria (ρ<α(0.05)) in almost all treatments. The conclusion of this research is that there is a difference in the antibacterial activity of 96% ethanol extract, ethyl acetate extract and n-hexane extract of papaya seeds on the growth of Sthaphylococcus aureus bacteria. Key words: papaya seeds, antibacterial test UJI AKTIVITAS ANTIBAKTERI Staphylococcus aureus BERDASARKAN PERBEDAAN JENIS PELARUT EKSTRAK BIJI BUAH PEPAYA (Carica papaya L.) ABSTRAK Staphylococcus aureus merupakan bakteri patogen opurtunistik permukaan kulit yang menyebabkan penyakit impetigo, terapi pengobatan penyakit impetigo dapat dilakukan dengan pemberian obat alami dari biji pepaya (Carica papaya L). Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri Staphylococcus aureus ekstrak biji pepaya berdasarkan perbedaan jenis pelarut ekstrak yang digunakan. Penelitian ini merupakan penelitian true experimental dengan bentuk penelitian posttest only control design. Metode penelitian yang digunakan adalah metode difusi cakram dengan tiga perlakuan ekstrak biji pepaya yaitu ekstrak etanol 96%, etil asetat dan n-heksana, kontrol positif menggunakan kloramfenikol dan kontrol negatif menggunakan etanol96%, etil asetat dan n-heksana. Hasil penelitian menunjukan masing-masing perlakuan ekstrak biji pepaya mampu menghambat Staphylococcus aureus dengan zona hambat ekstrak etanol96% 18,09 mm, ekstrak etil asetat 7,93 mm, ekstrak n-heksana 0,36 mm. Uji One Way Anova menunjukan nilai ρ<α(0,05) sehingga ada perbedaan signifikan pada diameter zona hambat disetiap perlakuan ekstrak. Uji Tukey menunjukan terdapat perbedaan bermakna zona hambat bakteri Staphylococcus aureus (ρ<α(0,05)) hampir diseluruh perlakuan. Simpulan penelitian ini adalah terdapat perbedaan aktivitas antibakteri ekstrak etanol 96%, ekstrak etil asetat dan ekstrak n-heksana biji buah pepaya terhadap pertumbuhan bakteri Staphylococcus aureus. Kata kunci : biji buah pepaya, uji antibakteri
| Item Type: | Thesis (Diploma) |
|---|---|
| Uncontrolled Keywords: | papaya seeds, antibacterial test biji buah pepaya, uji antibakteri |
| Subjects: | Q Science > Q Science (General) |
| Divisions: | Jurusan Analis Kesehatan |
| Depositing User: | STRTLM DENPASAR |
| Date Deposited: | 16 Jan 2025 00:49 |
| Last Modified: | 16 Jan 2025 00:49 |
| URI: | http://repository.poltekkes-denpasar.ac.id/id/eprint/14379 |
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