ANALISIS GEN LT DAN ST BAKTERI ENTEROTOXIGENIC ESCHERICHIA COLI DENGAN METODE POLYMERASE CHAIN REACTION PADA LAWAR PLEK DI DESA KETEWEL GIANYAR

Hardiyanta, I Made Yogi (2024) ANALISIS GEN LT DAN ST BAKTERI ENTEROTOXIGENIC ESCHERICHIA COLI DENGAN METODE POLYMERASE CHAIN REACTION PADA LAWAR PLEK DI DESA KETEWEL GIANYAR. Diploma thesis, Poltekkes Kemenkes Denpasar Jurusan Teknologi Laboratorium Medis 2024.

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Abstract

ANALYSIS OF LT AND ST GENES FROM Enterotoxigenic Escherichia coli BACTERIA USING POLYMERASE CHAIN REACTION METHOD ON LAWAR PLEK IN KETEWEL VILLAGE, GIANYAR ABSTRACT Lawar plek is a food made from raw meat. Raw meat potentially contain contaminant from pathogenic bacteria. One of the pathogenic bacteria is Enterotoxigenic Escherichia coli (ETEC). The purpose of this study is to identify Enterotoxigenic Escherichia coli bacteria using molecular method on lawar plek in Ketewel Village, Gianyar. This study use Eosin Methylene Blue Agar (EMBA) and continued by Polymerase Chain Reaction (PCR) as the identification method. The result form EMBA identification found 4 samples (33,33%) suspected colony of Escherichia coli bacteria and 8 samples (66,66%) neagitive. From that 4 samples, no virulence gene of LT (heat-labile toxin) and ST (heat-stable toxin) from Enterotoxigenic Escherichia coli are detected. From that results, the conclusion is that suspected Escherichia coli colony bacteria are found from the samples but

Item Type: Thesis (Diploma)
Uncontrolled Keywords: Enterotoxigenic Escherichia coli, lawar plek, polymerase chain reaction
Subjects: Q Science > QR Microbiology
Divisions: Jurusan Analis Kesehatan
Depositing User: STRTLM DENPASAR
Date Deposited: 13 Dec 2024 01:22
Last Modified: 13 Dec 2024 01:22
URI: http://repository.poltekkes-denpasar.ac.id/id/eprint/14205

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