Dewi, Kadek Eliana Kesuma (2019) UJI DAYA HAMBAT BERBAGAI KONSENTRASI PERASAN JERUK LEMON TERHADAP BAKTERI Propionibacterium acnes. Diploma thesis, Poltekkes Denpasar.
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Abstract
ABSTRACT Propionibacterium acnes is an bacteria that causes acne. One way to deal with acne is to use lemon. This study aims to determine the inhibition of various concentrations of lemon juice against growth of Propionibacterium acnes bacteria. This study was a true experiment with the Posttest Only Control Design, using Kirby Bauer diffusion disc method with six concentration (5, 12,5, 25, 50, 75, and 100%), work control (Chloramphenicol 30µg) and negative control (sterile aquadest). The results showed that lemon juice was able to inhibit the growth of Propionibacterium acnes bacteria with a mean inhibition zone of 7,15 mm (medium), 9,20 mm (medium), 11,25 mm (strong), 12,50 mm (strong), 14,10 mm (strong), and 16,90 mm (strong). Based on the One Way Anova test, it was found that the value of p (0,000) < α (0,05) showed that there was a difference in the diameter of the zone inhibiting the growth of Propionibacterium acnes bacteria in various concentration of lemon juice. The LSD test results showed that there were significant difference in the diameter of the inhibition zone between each concentration of lemon juice. Based on the results of the study it can be concluded that various concentration of lemon juice have different inhibition against Propionibacterium acnes bacteria. Keywords : lemon juice, Propionbacterium acnes, inhibition zone ABSTRAK Propionibacterium acnes merupakan bakteri yang menyebabkan terjadinya jerawat. Salah satu cara untuk mengatasi jerawat yaitu dengan memanfaatkan buah jeruk lemon. Penelitian ini bertujuan untuk mengetahui daya hambat berbagai konsentrasi perasan jeruk lemon terhadap pertumbuhan bakteri Propionibacterium acnes. Penelitian ini merupakan eksperimen murni dengan rancangan penelitian Posttest Only Control Design menggunakan metode difusi cakram Kirby Bauer dengan enam konsentrasi perasan buah jeruk lemon (5, 12,5, 25, 50, 75, dan 100%), kontrol kerja (Kloramfenikol 30µg), dan kontrol negatif (aquadest steril). Hasil penelitian menunjukkan bahwa perasan buah jeruk lemon mampu menghambat pertumbuhan bakteri Propionibacterium acnes dengan rerata zona hambat berturut-turut yaitu sebesar 7,15 mm (sedang), 9,20 mm (sedang), 11,25 mm (kuat), 12,50 mm (kuat), 14,10 mm (kuat), dan 16,90 mm (kuat). Berdasarkan uji One Way Anova diketahui bahwa nilai p (0,000) < α (0,05) yang menunjukkan ada perbedaan diameter zona hambat pertumbuhan bakteri Propionibacterium acnes pada berbagai konsentrasi perasan buah jeruk lemon. Hasil uji LSD menunjukkan bahwa terdapat perbedaan diameter zona hambat yang bermakna antara masing – masing konsentrasi perasan buah jeruk lemon. Berdasarkan hasil penelitian dapat disimpulkan bahwa berbagai konsentrasi perasan jeruk lemon memiliki perbedaan daya hambat terhadap bakteri Propionibacterium acnes. Kata kunci : perasan jeruk lemon, Propionibacterium acnes, zona hambat
| Item Type: | Thesis (Diploma) |
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| Uncontrolled Keywords: | Keywords : lemon juice, Propionbacterium acnes, inhibition zone Kata kunci : perasan jeruk lemon, Propionibacterium acnes, zona hambat |
| Subjects: | Q Science > QR Microbiology |
| Divisions: | Jurusan Analis Kesehatan > Prodi D3 |
| Depositing User: | mrs Kesuma Dewi Kadek Eliana |
| Date Deposited: | 02 Dec 2019 05:51 |
| Last Modified: | 02 Dec 2019 05:51 |
| URI: | http://repository.poltekkes-denpasar.ac.id/id/eprint/2915 |
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