Bela, Maria Putu Angelina (2026) PENGARUH SUHU DAN LAMA PENYIMPANAN URINE TERHADAP JUMLAH LEUKOSIT PADA PASIEN DENGAN SUSPECT INFEKSI SALURAN KEMIH. Diploma thesis, Poltekkes Kemenkes Denpasar Jurusan Teknologi Laboratorium Medis 2026.
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Abstract
THE EFFECT OF TEMPERATURE AND DURATION OF URINE STORAGE ON LEUKOCYTE COUNT IN PATIENTS WITH SUSPECTED URINARY TRACT INFACTION ABSTRACT Background: Urinary tract infections (UTIs) are one of the most common infectious diseases. Urine leukocyte examination is an important indicator in the diagnosis of UTIs; however, its reliability is influenced by pre-analytical factors such as temperature and specimen storage time. Objective: To determine the effect of temperature and storage time on urine leukocyte counts in patients with suspected UTIs. Methods: This study employed a true experimental design with a posttest-only control group. A total of 24 urine samples from 4 participants were examined using microscopic urine sediment analysis. Samples were stored at room temperature (25–27°C) and refrigerated temperature (4–8°C) for 0, 2, and 4 hours. Data analysis utilized the Shapiro-Wilk test, Levene’s test, Two-way ANOVA, and Tukey HSD post-hoc test. Results: Urine leukocyte counts decreased with increasing storage time, particularly at room temperature. The Two-way ANOVA indicated that storage temperature and duration significantly influenced urine leukocyte counts (p < 0.05). The greatest decrease occurred during the first 2 hours of storage. Conclusion: Urine storage temperature and duration affect the urine leukocyte count. Urinalysis should be performed within 2 hours of sample collection or stored at 4–8°C to ensure the accuracy of UTI test results. Keywords: UTI, Leukocytes, Temperature PENGARUH SUHU DAN LAMA PENYIMPANAN URINE TERHADAP JUMLAH LEUKOSIT PADA PASIEN DENGAN SUSPECT INFEKSI SALURAN KEMIH ABSTRAK Latar Belakang: Infeksi saluran kemih (ISK) merupakan salah satu penyakit infeksi yang sering ditemukan. Pemeriksaan leukosit urine menjadi indikator penting dalam diagnosis ISK, namun kestabilannya dipengaruhi oleh faktor pra analitik seperti suhu dan lama penyimpanan spesimen. Tujuan: Mengetahui pengaruh suhu dan lama penyimpanan terhadap jumlah leukosit urine pada pasien suspect ISK. Metode: Penelitian menggunakan desain true experimental dengan rancangan posttest-only control group. Sebanyak 24 sampel urine dari 4 responden diperiksa menggunakan metode sedimen urine secara mikroskopis. Sampel disimpan pada suhu ruang (25–27°C) dan suhu dingin (4–8°C) dengan variasi waktu 0 jam, 2 jam, dan 4 jam. Analisis data menggunakan uji Shapiro-Wilk, Levene, Two-way ANOVA, dan Post-hoc Tukey HSD. Hasil: Jumlah leukosit urine mengalami penurunan seiring bertambahnya waktu penyimpanan, terutama pada suhu ruang. Uji Two-way ANOVA menunjukkan bahwa suhu penyimpanan dan lama penyimpanan berpengaruh signifikan terhadap jumlah leukosit urine (p < 0,05). Penurunan terbesar terjadi pada 2 jam pertama penyimpanan. Simpulan: Suhu dan lama penyimpanan urine memengaruhi jumlah leukosit urine. Pemeriksaan urine sebaiknya dilakukan kurang dari 2 jam setelah pengambilan sampel atau disimpan pada suhu 4–8°C untuk menjaga keakuratan hasil pemeriksaan ISK. Kata Kunci: ISK, Leukosit, Suhu
| Item Type: | Thesis (Diploma) |
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| Uncontrolled Keywords: | UTI, Leukocytes, Temperature ISK, Leukosit, Suhu |
| Subjects: | Q Science > Q Science (General) R Medicine > R Medicine (General) |
| Divisions: | Jurusan Analis Kesehatan |
| Depositing User: | Paramita Ida Ayu Nia |
| Date Deposited: | 19 Oct 2026 05:49 |
| Last Modified: | 19 Oct 2026 05:49 |
| URI: | http://repository.poltekkes-denpasar.ac.id/id/eprint/23272 |
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