Artha Dewi, Ni Kadek Ayu Saraswati (2026) UJI AKTIVITAS ANTIBAKTERI KOMBUCHA TEH TELANG (Clitoria ternatea L.) BERDASARKAN PERBEDAAN WAKTU FERMENTASI TERHADAP BAKTERI Staphylococcus aureus. Diploma thesis, Poltekkes Kemenkes Denpasar Jurusan Teknologi Laboratorium Medis 2026.
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Abstract
ANTIBACTERIAL ACTIVITY TEST OF BUTTERFLY PEA FLOWER KOMBUCHA TEA (Clitoria ternatea L.) BASED ON FERMENTATION TIME DIFFERENCES AGAINST Staphylococcus aureus ABSTRACT Butterfly pea tea kombucha (Clitoria ternatea L.) has potential as an alternative antibacterial agent against Staphylococcus aureus due to its bioactive compound content, particularly amid the increasing bacterial resistance to antibiotics. This study aimed to analyze the effect of variations in fermentation time of butterfly pea tea kombucha on its antibacterial activity against Staphylococcus aureus. This research employed a true experimental method with a post-test only control group design. Antibacterial activity testing was conducted using the disk diffusion (Kirby-Bauer) method on Mueller Hinton Agar (MHA) media with fermentation time variations of 5, 7, 9, and 11 days. The results showed an increase in the average diameter of the inhibition zone along with longer fermentation time, namely 7.95 mm on day 5, 9.71 mm on day 7, 10.18 mm on day 9, and 11.64 mm on day 11. The positive control (chloramphenicol) produced an inhibition zone of 23.18 mm, while the negative control showed no inhibition zone. The One Way ANOVA test results showed a significance value of 0.000 (p < 0.05), indicating significant differences in antibacterial activity among each fermentation time variation. It can be concluded that butterfly pea tea kombucha has antibacterial activity against Staphylococcus aureus, with the highest activity observed on the 11th day of fermentation. The longer the fermentation time, the greater the diameter of the inhibition zone produced, indicating that fermentation duration affects the antibacterial effectiveness of butterfly pea tea kombucha. Keywords: antibacterial, kombucha, clitoria, fermentation, staphylococcus UJI AKTIVITAS ANTIBAKTERI KOMBUCHA TEH TELANG (Clitoria ternatea L.) BERDASARKAN PERBEDAAN WAKTU FERMENTASI TERHADAP BAKTERI Staphylococcus aureus ABSTRAK Kombucha teh telang (Clitoria ternatea L.) berpotensi sebagai agen antibakteri alternatif terhadap Staphylococcus aureus karena kandungan senyawa bioaktifnya, terutama di tengah meningkatnya resistensi bakteri terhadap antibiotik. Penelitian ini bertujuan menganalisis pengaruh variasi waktu fermentasi kombucha teh telang terhadap aktivitas antibakteri terhadap Staphylococcus aureus. Penelitian menggunakan metode true experimental dengan rancangan post-test only control group design. Uji aktivitas antibakteri dilakukan menggunakan metode difusi cakram (Kirby-Bauer) pada media Mueller Hinton Agar (MHA) dengan variasi waktu fermentasi hari ke-5, 7, 9, dan 11. Hasil penelitian menunjukkan adanya peningkatan rata-rata diameter zona hambat seiring bertambahnya lama fermentasi, yaitu pada hari ke-5 sebesar 7,95 mm, hari ke-7 sebesar 9,71 mm, hari ke-9 sebesar 10,18 mm, dan hari ke-11 sebesar 11,64 mm. Kontrol positif (kloramfenikol) menghasilkan zona hambat sebesar 23,18 mm, sedangkan kontrol negatif tidak menunjukkan zona hambat. Hasil uji One Way ANOVA menunjukkan nilai signifikansi 0,000 (p < 0,05), yang menunjukkan terdapat perbedaan signifikan aktivitas antibakteri pada setiap variasi waktu fermentasi. Disimpulkan bahwa kombucha teh telang memiliki aktivitas antibakteri terhadap Staphylococcus aureus, dengan aktivitas tertinggi pada fermentasi hari ke-11. Semakin lama waktu fermentasi, semakin besar diameter zona hambat yang dihasilkan, sehingga lama fermentasi berpengaruh terhadap efektivitas antibakteri kombucha teh telang. Kata kunci: antibakteri, kombucha, telang, fermentasi, staphylococcus
| Item Type: | Thesis (Diploma) |
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| Uncontrolled Keywords: | antibacterial, kombucha, clitoria, fermentation, staphylococcus antibakteri, kombucha, telang, fermentasi, staphylococcus |
| Subjects: | Q Science > Q Science (General) Q Science > QR Microbiology |
| Divisions: | Jurusan Analis Kesehatan |
| Depositing User: | Paramita Ida Ayu Nia |
| Date Deposited: | 15 Oct 2026 02:33 |
| Last Modified: | 15 Oct 2026 02:33 |
| URI: | http://repository.poltekkes-denpasar.ac.id/id/eprint/21017 |
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