Bryan, Surya Natallia (2026) PENGARUH KONSENTRASI EKSTRAK ETANOL DAUN SIRIH HIJAU (Piper betle linn) SEBAGAI ANTIBAKTERI TERHADAP PERTUMBUHAN BAKTERI Escherichia coli. Diploma thesis, Poltekkes Kemenkes Denpasar Jurusan Teknologi Laboratorium Medis 2026.
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Abstract
THE EFFECT OF THE CONCENTRATION OF ETHANOL EXTRACT OF GREEN BETEL LEAVES (Piper betle linn) AS AN ANTIBACTERIAL ON THE GROWTH OF Escherichia coli BACTERIA ABSTRACT Background: Green betel leaf (Piper betle Linn) is a traditional medicinal plant with potential antibacterial properties due to its secondary metabolite compounds. Purpose: This study aimed to determine the effect of ethanol extract concentration of green betel leaves as an antibacterial agent on the growth of Escherichia coli. Method: The method used was a true experimental design with a posttest-only control group design. The extract was obtained through maceration using 96% ethanol and prepared in concentrations of 20%, 40%, 60%, and 80%. The antibacterial activity test was conducted using the well diffusion method on Mueller Hinton Agar (MHA) by measuring the diameter of the inhibition zones. Result: Phytochemical screening results indicated the presence of saponins, tannins, and terpenoids. The average inhibition zone diameters were 17.6 mm, 17.8 mm, 18.2 mm, and 19.7 mm, respectively, which were categorized as strong. The positive control (levofloxacin) showed very strong antibacterial activity (25.1 mm), while the negative control showed no inhibition. Statistical analysis using One Way ANOVA (Welch) showed a significant difference among treatment groups (p < 0.05), while the Games-Howell post hoc test indicated that not all increases in concentration were significantly different. Conlucision: In conclusion, the concentration of ethanol extract of green betel leaf (Piper betle Linn) affects its antibacterial activity against Escherichia coli, with higher concentrations tending to increase the inhibition zone diameter and the optimal concentration ranging from 60% to 80%. Keyword: green betel leaf, Escherichia coli. PENGARUH KONSENTRASI EKSTRAK ETANOL DAUN SIRIH HIJAU (Piper betle linn) SEBAGAI ANTIBAKTERI TERHADAP PERTUMBUHAN BAKTERI Escherichia coli ABSTRAK Latar Belakang: Daun sirih hijau (Piper betle linn) merupakan tanaman obat tradisional yang berpotensi sebagai antibakteri karena mengandung senyawa metabolit sekunder. Tujuan: Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi ekstrak etanol daun sirih hijau (Piper betle linn) sebagai antibakteri terhadap pertumbuhan bakteri Escherichia coli. Metode: Metode yang digunakan adalah true experimental dengan rancangan posttest only control group design. Ekstrak diperoleh melalui maserasi menggunakan etanol 96% dan dibuat dalam konsentrasi 20%, 40%, 60%, dan 80%. Uji aktivitas antibakteri dilakukan dengan metode difusi sumuran pada media Mueller Hinton Agar (MHA) dengan mengukur diameter zona hambat. Hasil: Hasil skrining fitokimia menunjukkan adanya saponin, tanin, dan terpenoid. Rata-rata diameter zona hambat berturut-turut adalah 17,6 mm; 17,8 mm; 18,2 mm; dan 19,7 mm yang tergolong kuat. Kontrol positif (levofloxacin) menunjukkan aktivitas sangat kuat (25,1 mm), sedangkan kontrol negatif tidak menunjukkan hambatan. Analisis One Way ANOVA (Welch) menunjukkan perbedaan signifikan (p<0,05), namun uji Games-Howell menunjukkan tidak semua peningkatan konsentrasi berbeda signifikan. Simpulan: Berdasarkan hasil penelitian, dapat disimpulkan bahwa konsentrasi ekstrak etanol daun sirih hijau (Piper betle linn) berpengaruh terhadap aktivitas antibakteri terhadap pertumbuhan Escherichia coli. Peningkatan konsentrasi cenderung meningkatkan diameter zona hambat dengan konsentrasi optimal 60%–80%. Kata kunci: Daun sirih hijau, Escherichia coli.
| Item Type: | Thesis (Diploma) |
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| Uncontrolled Keywords: | green betel leaf, Escherichia coli. Daun sirih hijau, Escherichia coli. |
| Subjects: | Q Science > Q Science (General) Q Science > QR Microbiology |
| Divisions: | Jurusan Analis Kesehatan |
| Depositing User: | Paramita Ida Ayu Nia |
| Date Deposited: | 19 Oct 2026 04:44 |
| Last Modified: | 19 Oct 2026 04:44 |
| URI: | http://repository.poltekkes-denpasar.ac.id/id/eprint/20670 |
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