UJI AKTIVITAS ANTIBAKTERI NANOEMULSI EKSTRAK AKAR BAMA (Plumbago zeylanica L) DAN VCO (Virgin Coconut Oil) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus DAN Cutibacterium acnes

Indraswari, Putu Inten Ishita (2026) UJI AKTIVITAS ANTIBAKTERI NANOEMULSI EKSTRAK AKAR BAMA (Plumbago zeylanica L) DAN VCO (Virgin Coconut Oil) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus DAN Cutibacterium acnes. Diploma thesis, Poltekkes Kemenkes Denpasar Jurusan Teknologi Laboratorium Medis 2026.

Preview	Text (Skripsi 2026) HALAMAN DEPAN.pdf Download (722kB) | Preview
Preview	Text (Skripsi 2026) BAB I PENDAHULUAN.pdf Download (290kB) | Preview
Preview	Text (Skripsi 2026) BAB II TINJAUAN PUSTAKA (2).pdf Download (507kB) | Preview
Preview	Text (Skripsi 2026) BAB III KERANGKA KONSEP.pdf Download (333kB) | Preview
Preview	Text (Skripsi 2026) BAB IV METODE PENELITIAN.pdf Download (402kB) | Preview
	Text (Skripsi 2026) BAB V HASIL DAN PEMBAHASAN (11).pdf Restricted to Repository staff only Download (805kB)
Preview	Text (Skripsi 2026) BAB VI PENUTUP.pdf Download (274kB) | Preview
Preview	Text (Skripsi 2026) DAFTAR PUSTAKA.pdf Download (269kB) | Preview
	Text (Skripsi 2026) LAMPIRAN-LAMPIRAN (2).pdf Restricted to Repository staff only Download (2MB)

Abstract

ANTIBACTERIAL ACTIVITY TEST OF BAMA ROOT (Plumbago zeylanica L.) EXTRACT NANOEMULSION AND VCO (Virgin Coconut Oil) AGAINST THE GROWTH OF Staphylococcus aureus AND Cutibacterium acnes ABSTRACT Background: Acne is a chronic inflammatory skin disease associated with the growth of Staphylococcus aureus and Cutibacterium acnes. Long-term use of antibiotics may lead to bacterial resistance; therefore, natural antibacterial alternatives are needed. Bama root (Plumbago zeylanica L.) contains bioactive compounds with potential antibacterial properties. This study aimed to determine the antibacterial activity of bama root extract nanoemulsion and virgin coconut oil (VCO) against the growth of Staphylococcus aureus and Cutibacterium acnes. Methods: This study was a true experimental research with a posttest only control group design. Antibacterial activity was tested using the disc diffusion method on three formulas: F1 (0.1%), F2 (0.5%), and F3 (1.0%), along with positive and negative controls. Data were analyzed using Shapiro-Wilk, Kruskal-Wallis, and Post Hoc Games-Howell tests. Results: Formula F1 showed no inhibition zone against both bacteria. Formula F2 produced a mean inhibition zone diameter of 9.7 ±0.38 mm against Staphylococcus aureus and showed no inhibition against Cutibacterium acnes. Formula F3 produced mean inhibition zone diameters of 15.6± 0.37 mm against Staphylococcus aureus and 26.5± 1.03 mm against Cutibacterium acnes. Kruskal-Wallis test showed p=0.000 (p<0.05) for both bacteria, indicating significant differences among formulas. Conclusion: Bama root extract nanoemulsion and VCO have antibacterial activity, with formula F3 (1%) as the most effective concentration against Staphylococcus aureus and Cutibacterium acnes. Keywords: Plumbago zeylanica L, Nanoemulsion, Virgin Coconut Oil, Antibacterial UJI AKTIVITAS ANTIBAKTERI NANOEMULSI EKSTRAK AKAR BAMA (Plumbago zeylanica L) dan VCO (Virgin Coconut Oil) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus DAN Cutibacterium acnes ABSTRAK Latar belakang: Jerawat merupakan penyakit kulit inflamasi kronik yang berkaitan dengan pertumbuhan bakteri Staphylococcus aureus dan Cutibacterium acnes. Penggunaan antibiotik jangka panjang berisiko menimbulkan resistensi, sehingga diperlukan alternatif antibakteri berbahan alam. Akar bama (Plumbago zeylanica L.) mengandung senyawa bioaktif yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri nanoemulsi ekstrak akar bama dan Virgin Coconut Oil (VCO) terhadap pertumbuhan Staphylococcus aureus dan Cutibacterium acnes. Metode: Jenis penelitian ini merupakan true experimental dengan rancangan posttest only control group design. Uji antibakteri menggunakan metode difusi cakram pada formula F1 (0,1%), F2 (0,5%), dan F3 (1,0%), serta kontrol positif dan negatif. Data dianalisis menggunakan uji Shapiro-Wilk, Kruskal-Wallis, dan Post Hoc Games-Howell. Hasil: Nanoemulsi formula F1 tidak menunjukkan zona hambat pada kedua bakteri. Formula F2 menghasilkan rerata diameter zona hambat 9,7± 0,38 mm terhadap Staphylococcus aureus dan tidak menghambat Cutibacterium acnes. Formula F3 menghasilkan rerata diameter zona hambat 15,6± 0,37 mm terhadap Staphylococcus aureus dan 26,5 ± 1,03 mm terhadap Cutibacterium acnes. Uji Kruskal-Wallis menunjukkan nilai p=0,000 (p<0,05) pada kedua bakteri, yang berarti terdapat perbedaan signifikan antar formula. Simpulan: Nanoemulsi ekstrak akar bama dan VCO memiliki aktivitas antibakteri, dengan formula F3 (1%) sebagai konsentrasi paling efektif terhadap Staphylococcus aureus dan Cutibacterium acnes. Kata kunci: Akar Plumbago zeylanica L, Nanoemulsi, Virgin Coconut Oil, Antibakteri.

Actions (login required)

View Item