ANALISIS KUALITAS PULASAN DAN MOUNTING DARI SEDIAAN BLOK PARAFIN PASIEN KANKER SERVIKS DI RSUD KABUPATEN KLUNGKUNG

Chandra, Ni Kadek Suryaning (2026) ANALISIS KUALITAS PULASAN DAN MOUNTING DARI SEDIAAN BLOK PARAFIN PASIEN KANKER SERVIKS DI RSUD KABUPATEN KLUNGKUNG. Diploma thesis, Poltekkes Kemenkes Denpasar Jurusan Teknologi Laboratorium Medis 2026.

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Abstract

ANALYSIS OF THE QUALITY OF STAINING AND MOUNTING OF PARAFFIN BLOCK PREPARATIONS FROM CERVICAL CANCER PATIENTS AT KLUNGKUNG DISTRICT GENERAL HOSPITAL ABSTRACT Cervical cancer is one of the major health problems among women, with incidence and mortality rates that remain high worldwide as well as in Indonesia. The diagnosis of cervical cancer can be established through histopathological examination using biopsy tissue processed into paraffin block preparations. The quality of histopathological slides is greatly influenced by the preparation stages, one of which is the staining and mounting process. This study was conducted to determine the quality of staining and mounting of paraffin block slides from cervical cancer patients. This was a descriptive study conducted on 18 paraffin blocks from cervical cancer patients obtained through total sampling at Klungkung Regional General Hospital. The paraffin blocks were sectioned using a microtome into thin slides, which were then stained with hematoxylin-eosin (HE) and mounted using Entellan. Univariate analysis showed that the quality of staining and mounting of histopathological slides in the good category was found in 9 slides (50.0%). However, there were still variations in slides categorized as fair and poor quality. The analysis of staining and mounting quality of paraffin block slides from cervical cancer patients showed that 9 slides (50.0%) were categorized as good. Therefore, slides in this category were still suitable to be used as supporting materials for establishing diagnosis by pathologists. Keywords: Hematoxylin-eosin staining, Mounting, Cervical Cancer ANALISIS KUALITAS PULASAN DAN MOUNTING DARI SEDIAAN BLOK PARAFIN PASIEN KANKER SERVIKS DI RSUD KABUPATEN KLUNGKUNG ABSTRAK Kanker serviks merupakan salah satu masalah kesehatan pada perempuan dengan angka kejadian dan kematian yang masih tinggi, baik di dunia maupun di Indonesia. Diagnosis kanker serviks dapat ditegakkan melalui pemeriksaan histopatologi menggunakan jaringan biopsi yang diproses menjadi sediaan blok parafin. Kualitas sediaan histopatologi sangat dipengaruhi oleh tahapan preparasi, salah satunya adalah proses pulasan dan mounting. Penelitian dilakukan untuk mengetahui kualitas pulasan dan mounting sediaan blok parafin pasien kanker serviks. Penelitian deskriptif pada 18 blok parafin pasien kanker serviks yang diperoleh secara total sampling di RSUD Kabupaten Klungkung. Blok parafin pasien kanker serviks dipotong menggunakan mikrotom sehingga menjadi sediaan tipis yang akan diwarnai dengan hematoksilin-eosin (HE) dan mounting dengan entelan. Analisis univariat menunjukkan bahwa kualitas pulasan dan mounting sediaan histopatologi dengan kategori baik yaitu sebanyak 9 sediaan (50,0%). Namun, masih terdapat variasi sediaan yang berkualitas cukup dan buruk. Analisis kualitas pulasan dan mounting sediaan blok parafin pasien kanker serviks menunjukkan bahwa kategori baik diperoleh sebanyak 9 sediaan (50,0%). Dengan demikian, sediaan pada kategori tersebut masih layak digunakan sebagai penunjang penegakan diagnosis oleh dokter. Kata Kunci: Pewarnaan hematoksilin-eosin, Mounting, Kanker serviks.

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