POCKET MEASUREMENT METHODS IN WISTAR RATS PERIODONTITIS INDUCED BY BACTERIA AND THE INSTALLATION OF SILK LIGATURE: AN EXPERIMENTAL STUDIES

Dharmawati, I Gusti Agung Ayu POCKET MEASUREMENT METHODS IN WISTAR RATS PERIODONTITIS INDUCED BY BACTERIA AND THE INSTALLATION OF SILK LIGATURE: AN EXPERIMENTAL STUDIES. International Journal of Applied Pharmaceutics, 11 (4). ISSN 0975-7058

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Abstract

Objective: The purpose of this study was to develop an experimental model of induction of periodontal disease in Wistar rats, using a combination of bacterial induction and binding of silk ligature with respect to pocket periodontal depth. Methods: Experiments with a pre-posttest group design was applied. Five adult male Wistar rats from the Udayana University's Analytical Laboratory were included in the study. Measuring pocket depth in experimental animals using dental probes was previously administered. Then performed the installation of silk ligature and bacterial induction Porphyromonas gingivalis, on the mandibular anterior teeth. Release of silk ligature on day 7, without the action of debridement of plaque or calculus on rat tooth. Observation of the development of the testing animals on the 3d, 7d, and 11d. On the 11d, re-measurement of pocket depth was conducted. Results: Periodontal tissue abnormalities with silk ligature placement and bacterial infiltration Porphyromonas gingivalis cause periodontal inflammation with periodontal pocket formation, with a mean depth of 3.32 mm, which was analyzed using Wilcoxon p<0.05. Conclusion: In this study combining bacterial induction and the installation of silk ligature can shorten the induction of periodontal tissue disease characterized by the formation of pocket periodontal.

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