UJI AKTIVITAS ANTIBAKTERI REBUSAN DAUN SIRIH (Piper betle Linn) TERHADAP BAKTERI Streptococcus pyogenes

AMANDA, SEVITA (2018) UJI AKTIVITAS ANTIBAKTERI REBUSAN DAUN SIRIH (Piper betle Linn) TERHADAP BAKTERI Streptococcus pyogenes. Diploma thesis, JURUSAN ANALIS KESEHATAN.

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Abstract

Background: Infection is a disease caused by pathogenic microbes and is very dynamic. ISPA is defined as an acute respiratory disease caused by infectious agents that were transmitted from human to human. One of the microbes that caused ISPA is Streptococcus pyogenes, which is a gram-positive bacteria and belongs to Streptococcus sp. â-hemolytic class A. Nowadays, many people are begin to use natural materials to deal with microbial infections. One of them is the betel leaf (Piper betle Linn.) Which has antibacterial and antibiotic components for the treatment of respiratory problems, and bacterial infectious diseases. Purpose: This research was conducted with the aim to know the antibacterial activity of betel leaves (Piper betle Linn) decoction against Streptococcus pyogenes bacteria. Methods: This research used true experimental design with Posttest Only Control Design, and data analysis with One Way Anova and Least Significant Deference (LSD). Betel leaves decoction water is made in concentrations of 20%, 40%, 60%, 80%, and 100%, then tested on Streptococcus pyogenes bacteria with two replicates and four repetitions. Result: From a total of 40 data obtained, the mean diameter of the inhibitory zone at each concentrations is 20% 0 mm, 40% 12.9 mm, 60% 22.7 mm, 80% 25.5 mm, and 100% 30 mm. Conclution: Betel leaves decoction water is able to inhibit the growth of Streptococcus pyogenes with significant difference of drag zone diameter at each concentration. Keywords: Antibacterial, Betel Leaves, Streptococcus pyogenes Latar belakang: Infeksi adalah penyakit yang disebabkan oleh mikroba patogen dan bersifat sangat dinamis. ISPA didefinisikan sebagai penyakit saluran pernapasan akut disebabkan oleh agen infeksius yang ditularkan dari manusia ke manusia. Salah satu mikroba penyebab ISPA adalah Streptococcus pyogenes, yang merupakan bakteri gram positif dan tergolong dalam Streptococcus sp. â-hemolytic golongan A. Dewasa ini, banyak masyarakat yang mulai memanfaatkan bahan alam untuk menangani infeksi akibat mikroba. Salah satunya adalah daun sirih (Piper betle Linn.) yang memiliki komponen antibakteri dan antibiotik untuk pengobatan masalah pernapasan, dan penyakit akibat infeksi bakteri. Tujuan: Penelitian ini dilakukan dengan tujuan untuk mengetahui aktivitas antibakteri rebusan daun sirih (Piper betle Linn) terhadap bakteri Streptococcus pyogenes. Metode: Penelitian ini menggunakan jenis penelitian true experimental design dengan Posttest Only Control Design, dan analisis data dengan One Way Anova serta Least Significant Deference (LSD). Air rebusan daun sirih dibuat dalam konsentrasi 20%, 40%, 60%, 80%, dan 100%, kemudian diuji pada bakteri Streptococcus pyogenes dengan dua replikasi dan empat kali pengulangan. Hasil: Dari total 40 data yang didapat, nilai rerata diameter zona hambat yaitu pada konsentrasi 20% 0 mm, 40% 12,9 mm, 60% 22,7 mm, 80% 25,5 mm, dan 100% 30 mm. Simpulan: Air rebusan daun sirih mampu menghambat pertumbuhan Streptococcus pyogenes dengan perbedaan diameter zona hambat yang bermakna pada masing-masing konsentrasi. Kata kunci: Antibakteri, Daun Sirih, Streptococcus pyogenes

Item Type: Thesis (Diploma)
Additional Information: BAKTERI Streptococcus pyogenes
Uncontrolled Keywords: ANTI BAKTERI
Subjects: Q Science > QR Microbiology
R Medicine > R Medicine (General)
Divisions: Jurusan Analis Kesehatan > Prodi D3
Depositing User: Sevita Amanda
Date Deposited: 31 Jul 2018 06:59
Last Modified: 31 Jul 2018 06:59
URI: http://repository.poltekkes-denpasar.ac.id/id/eprint/410

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