Proteomic study of bioactive peptides from tempe

Tamam, Dr. Badrut (2019) Proteomic study of bioactive peptides from tempe. [Dataset]

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Abstract

13 Tempe is a traditional Indonesian fermented soybean mostly produced in small industries and sold locally throughout 79 14 the country. Studies on the bioactive peptides in tempe are rare. Here, we studied bioactive peptides in samples from 80 15 three tempe producers with different degrees of sanitation. The peptide sub-fractions of tempe from each producer 81 16 were collected following water extraction, ultrafiltration (<3 kDa), gel filtration chromatography, and reversed phase- 82 high performance liquid chromatography (RP-HPLC) separation followed by LC-MS. The MS spectra were then pre- 17 dicted using FindPept tools (https://web.expasy.org/findpept/), and their biofunctionalities were confirmed with BIOPEP 83 18 databases (www.uwm.edu.pl/biochemia/index.php/pl/biopep). There were few similar peptides found in tempe from the 84 19 three producers. Peptides Val-His and Ala-Leu-Glu-Pro were found in tempe from all producers. Producers having a good 85 20 sanitation level had more bioactive peptides than those with moderate or poor sanitation levels (58%, 43% and 35%, 86 from good to poor sanitation). This work showed that the tempe from the three producers had antihypertensive, 21 antidiabetic, antioxidative and antitumor peptides.

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