Tamam, Dr. Badrur and Syah, Dahrul and Suhartono, Maggy Thenawidjaja and Kusuma, Wisnu Ananta and Tachibana, Shinjiro and Lioe, Hanifah Nuryani (2019) Proteomic study of bioactive peptides from tempe. Journal of Bioscience and Bioengineering, VOL. x (xxx). pp. 1-8. ISSN 0xxxx
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Abstract
Tempe is a traditional Indonesian fermented soybean mostly produced in small industries and sold locally throughout the country. Studies on the bioactive peptides in tempe are rare. Here, we studied bioactive peptides in samples from three tempe producers with different degrees of sanitation. The peptide sub-fractions of tempe from each producer were collected following water extraction, ultrafiltration (<3 kDa), gel filtration chromatography, and reversed phase-high performance liquid chromatography (RP-HPLC) separation followed by LC-MS. The MS spectra were then pre- 17 dicted using FindPept tools (https://web.expasy.org/findpept/), and their biofunctionalities were confirmed with BIOPEP databases (www.uwm.edu.pl/biochemia/index.php/pl/biopep). There were few similar peptides found in tempe from the three producers. Peptides Val-His and Ala-Leu-Glu-Pro were found in tempe from all producers. Producers having a good sanitation level had more bioactive peptides than those with moderate or poor sanitation levels (58%, 43% and 35%,from good to poor sanitation). This work showed that the tempe from the three producers had antihypertensive,antidiabetic, antioxidative and antitumor peptides.
| Item Type: | Article |
|---|---|
| Additional Information: | Tempe |
| Uncontrolled Keywords: | Proteomic study |
| Subjects: | R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine S Agriculture > S Agriculture (General) |
| Divisions: | Jurusan Gizi > Prodi D4 |
| Depositing User: | Dr Badrut Tamam |
| Date Deposited: | 16 Jun 2020 06:26 |
| Last Modified: | 28 Jan 2022 03:24 |
| URI: | http://repository.poltekkes-denpasar.ac.id/id/eprint/4070 |
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Proteomic study of bioactive peptides from tempe. (deposited 16 Jun 2020 00:32)
- Proteomic study of bioactive peptides from tempe. (deposited 16 Jun 2020 06:26) [Currently Displayed]
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