Effect of Nigella sativa Ethanol Extract on the Nitric Oxide Content and Renal Arteriole Diameter of a Pre-eclampsia Mouse Model

Purnamayanti, Ni Made Dwi and Windu, Siti Candra and Poeranto, Sri (2018) Effect of Nigella sativa Ethanol Extract on the Nitric Oxide Content and Renal Arteriole Diameter of a Pre-eclampsia Mouse Model. The Eurasian Journal of Medicine, 50 (3). pp. 148-151.

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Abstract

Objective: The aim of this study was to investigate the effect of Nigella sativa ethanol extract on nitric oxide (NO) levels and renal arteriole diameter of a pre-eclampsia mouse model. Materials and Methods: This experimental study was conducted using a post-test only control group design. Thirty BALB/c mice were divided into six groups: one negative control (normal pregnant mice), one positive control (pre-eclampsia model), and four groups of pre-eclampsia mice treated with varying doses of N. sativa (500 mg/kg body weight (BW)/day, 1000 mg/kg BW/day, 1500 mg/kg BW/day, and 2000 mg/kg BW/day). Ethanol extract of N. sativa was given for 5 days. Data were analyzed using analysis of variance. Results: We detected significant differences in NO levels between the pre-eclampsia mouse model and those given the ethanol extract. The latter had NO levels of 85.77±4.47 µM (500 mg/kg BW/day), 189.04±6.01 µM (1000 mg/kg BW/day), 226.56±2.13 µM (1500 mg/BW/day), and 207.98±4.74 µM (2000 mg/kg BW/day). The mean renal arteriole diameter showed significant differences among the treatment groups with N. sativa doses of 1000, 1500, and 2000 mg (15.15±2.21b μm, 16.35±2.52b μm, and 15.76±3.03b µm, respectively). Conclusion: N. sativa ethanol extract treatment increases NO levels and enlarges renal arteriole diameter of a pre-eclampsia mouse model in a dose-dependent manner. Keywords: Nitric oxide, Nigella sativa, pre-eclampsia, renal arteriole diameter

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