PERBEDAAN DAYA HAMBAT EKSTRAK ETANOL DAUN JUWET (Syzygium cumini L.) DENGAN VARIASI KONSENTRASI TERHADAP PERTUMBUHAN BAKTERI Methicillin Resistant Staphylococcus aureus

Santika Dewi, I Gusti Ayu (2019) PERBEDAAN DAYA HAMBAT EKSTRAK ETANOL DAUN JUWET (Syzygium cumini L.) DENGAN VARIASI KONSENTRASI TERHADAP PERTUMBUHAN BAKTERI Methicillin Resistant Staphylococcus aureus. Diploma thesis, Poltekkes Denpasar.

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Abstract

THE DIFFERENTIATION OF INHIBITORY POTENTIAL OF JUWET LEAVES ETHANOL EXTRACT (Syzygium cumini L.) WITH VARIOUS CONCENTRATION TOWARDS THE GROWTH OF Methicillin Resistant Staphylococcus aureus ABSTRACT Methicillin Resistant Staphylococcus aureus are pathogen bacterias that causes infections that experiences antibiotic resistance. Juwet leaves are known to have antibacterial properties such as, alkaloid, fenol, saponin and steroid. This experiment’s purpose was to know the difference in inhibition of juwet leaves ethanol extract with variations in concentration towards the growth of Methicillin Resistant Staphylococcus aureus. This research was a true experiment designed with Posttest Only Control Group Design, using Kirby-Bauer diffusion discs method on four concentrations (20%, 40%, 60%, and 80%) work controls (ciprofloxacin 5 μg) and negative control (96% ethanol). Results showed that the juwet leaf ethanol extract is able to inhibit Methicillin Resistant Staphylococcus aureus. with inhibition diameter zone of 80% (12,15 mm), 60% (11,18 mm), 40% (9,71 mm), 20% (7,70 mm), and negative control (0 mm). One Way Anova testing shows the value of p < α (0,05) which means there are differences in growth inhibition zone of Methicillin Resistant Staphylococcus aureus on various concentration of juwet leaf ethanol extract. In conclusion, this experiment provides that there is a difference in inhibition of juwet leaf ethanol extract with variations in concentration towards the growth of Methicillin Resistant Staphylococcus aureus. Keywords: Inhibition zone, Juwet leaf ethanol extract, Methicillin Resistant Staphylococcus aureus PERBEDAAN DAYA HAMBAT EKSTRAK ETANOL DAUN JUWET (Syzygium cumini L.) DENGAN VARIASI KONSENTRASI TERHADAP PERTUMBUHAN BAKTERI Methicillin Resistant Staphylococcus aureus ABSTRAK Methicillin Resistant Staphylococcus aureus merupakan bakteri pathogen penyebab infeksi yang mengalami resistensi terhadap antibiotik. Daun juwet memiliki senyawa antibakteri seperti alkaloid, fenol, saponin dan steroid. Penelitian ini bertujuan untuk mengetahui perbedaan daya hambat ekstrak etanol daun juwet (Syzygium cumini L.) dengan variasi konsentrasi terhadap pertumbuhan bakteri Methicillin Resistant Staphylococcus aureus. Penelitian ini merupakan true experiment dengan rancangan Posttest Only Control Group Design menggunakan metode difusi cakram Kirby-Bauer dengan empat konsentrasi (20%, 40%, 60% dan 80%), kontrol kerja (ciprofloxacin 5 μg) dan kontrol negatif (etanol 96%). Hasil penelitian menunjukkan, ekstrak etanol daun juwet mampu menghambat Methicillin Resistant Staphylococcus aureus dengan diameter zona hambat 80% (12,15 mm), 60% (11,18 mm), 40% (9,71 mm), 20% (7,70 mm), dan kontrol negatif (0 mm). Uji One Way Anova menunjukkan nilai p < α(0,05) sehingga ada perbedaan zona hambat pertumbuhan Methicillin Resistant Staphylococcus aureus pada berbagai konsentrasi ekstrak etanol daun juwet. Simpulan penelitian ini adalah ada perbedaan daya hambat ekstrak etanol daun juwet dengan variasi konsentrasi terhadap pertumbuhan bakteri Methicillin Resistant Staphylococcus aureus. Kata kunci: ekstrak etanol daun juwet; Methicillin Resistant Staphylococcus aureus; zona hambat.

Item Type: Thesis (Diploma)
Uncontrolled Keywords: Keywords: Inhibition zone, Juwet leaf ethanol extract, Methicillin Resistant Staphylococcus aureus Kata kunci: ekstrak etanol daun juwet; Methicillin Resistant Staphylococcus aureus; zona hambat.
Subjects: Q Science > QR Microbiology
T Technology > T Technology (General)
Divisions: Jurusan Analis Kesehatan > Prodi D3
Depositing User: mrs Santika Dewi I Gusti Ayu
Date Deposited: 29 Jun 2020 04:23
Last Modified: 29 Jun 2020 04:23
URI: http://repository.poltekkes-denpasar.ac.id/id/eprint/2904

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