Maylena, Desak Ayu Putu Trishna (2026) UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL PEGAGAN (CENTELLA ASIATICA) TERHADAP PROPIONIBACTERIUM ACNES. Diploma thesis, Poltekkes Kemenkes Denpasar Jurusan Teknologi Laboratorium Medis 2026.
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Abstract
ANTIBACTERIAL ACTIVITY TEST OF ETHANOL EXTRACT OF GOTU KOLA (Centella asiatica) AGAINST Propionibacterium acnes ABSTRACT Background: Acne vulgaris is a chronic skin disease caused by Propionibacterium acnes infection, with a prevalence of 85–100% among Indonesian adolescents. Prolonged use of conventional antibiotics such as clindamycin leads to bacterial resistance, necessitating natural-based alternatives. Gotu kola (Centella asiatica) contains flavonoids, saponins, tannins, and triterpenoids with potential antibacterial properties. Objective: To determine the antibacterial activity and the most effective concentration of C. asiatica ethanol extract against Propionibacterium acnes. Methods: A laboratory experimental study with a Posttest Only Control Group Design using the Kirby-Bauer disc diffusion method on MHA medium. The extract was obtained by maceration with 96% ethanol and tested at 10%, 20%, 30%, and 50% concentrations. Chloramphenicol served as the positive control and 96% ethanol as the negative control. Data were analyzed using ANOVA and LSD tests. Results: Mean inhibition zone diameters at 10%, 20%, 30%, and 50% were 5.33 mm, 5.65 mm, 6.42 mm, and 7.24 mm, respectively — all categorized as moderate inhibition (5–10 mm). The positive control yielded 31.0 mm. ANOVA showed significant differences between groups (p < 0.05). Conclusion: Ethanol extract of Centella asiatica exhibits antibacterial activity against Propionibacterium acnes, with the 50% concentration being the most effective (inhibition zone 7.24 mm). Keywords: Centella asiatica; Antibacterial; Propionibacterium acnes UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL PEGAGAN (Centella asiatica) TERHADAP Propionibacterium acnes ABSTRAK Latar Belakang: Jerawat (acne vulgaris) merupakan penyakit kulit kronis akibat infeksi Propionibacterium acnes yang prevalensinya mencapai 85–100% pada remaja Indonesia. Penggunaan antibiotik konvensional seperti klindamisin secara jangka panjang memicu resistensi bakteri, sehingga diperlukan alternatif berbasis bahan alam. Pegagan (Centella asiatica) mengandung flavonoid, saponin, tanin, dan triterpenoid yang berpotensi sebagai antibakteri. Tujuan: Mengetahui aktivitas antibakteri dan konsentrasi paling efektif ekstrak etanol pegagan terhadap Propionibacterium acnes. Metode: Penelitian eksperimen laboratorium dengan desain Posttest Only Control Group Design menggunakan metode difusi cakram Kirby-Bauer pada media MHA. Ekstrak diperoleh melalui maserasi etanol 96% dan diuji pada konsentrasi 10%, 20%, 30%, dan 50%. Kloramfenikol sebagai kontrol positif dan etanol 96% sebagai kontrol negatif. Data dianalisis dengan uji ANOVA dan LSD. Hasil: Rerata diameter zona hambat pada konsentrasi 10%, 20%, 30%, dan 50% berturut-turut adalah 5,33 mm, 5,65 mm, 6,42 mm, dan 7,24 mm, seluruhnya berkategori sedang (5–10 mm). Kontrol positif menghasilkan zona hambat 31,0 mm. Uji ANOVA menunjukkan perbedaan signifikan antar perlakuan (p < 0,05). Simpulan: Ekstrak etanol pegagan terbukti memiliki aktivitas antibakteri terhadap Propionibacterium acnes dengan konsentrasi 50% sebagai yang paling efektif (zona hambat 7,24 mm). Kata kunci: Pegagan; Antibakteri; Propionibacterium acnes
| Item Type: | Thesis (Diploma) |
|---|---|
| Subjects: | Q Science > QR Microbiology S Agriculture > S Agriculture (General) |
| Divisions: | Jurusan Analis Kesehatan |
| Depositing User: | Paramita Ida Ayu Nia |
| Date Deposited: | 23 Oct 2026 00:49 |
| Last Modified: | 23 Oct 2026 00:49 |
| URI: | http://repository.poltekkes-denpasar.ac.id/id/eprint/23981 |
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