IDENTIFIKASI METHICILLIN RESISTANT Staphylococcus aureus (MRSA) PADA TANGAN PETUGAS KEBERSIHAN DI TPS 3R KUBU LESTARI

Putriningsih, Ni Luh (2026) IDENTIFIKASI METHICILLIN RESISTANT Staphylococcus aureus (MRSA) PADA TANGAN PETUGAS KEBERSIHAN DI TPS 3R KUBU LESTARI. Diploma thesis, Poltekkes Kemenkes Denpasar Jurusan Teknologi Laboratorium Medis 2026.

	Text (Karya Tulis Ilmiah 2026 D-III TLM) Halaman Depan.pdf Download (689kB)
	Text (Karya Tulis Ilmiah 2026 D-III TLM) BAB I Pendahuluan.pdf Download (269kB)
	Text (Karya Tulis Ilmiah 2026 D-III TLM) BAB II Tinjauan Pustaka.pdf Download (365kB)
	Text (Karya Tulis Ilmiah 2026 D-III TLM) BAB III Kerangka Konsep.pdf Download (276kB)
	Text (Karya Tulis Ilmiah 2026 D-III TLM) BAB IV Metode Penelitian.pdf Download (349kB)
	Text (Karya Tulis Ilmiah 2026 D-III TLM) BAB V Hasil dan Pembahasan.pdf Restricted to Repository staff only Download (567kB) | Request a copy
	Text (Karya Tulis Ilmiah 2026 D-III TLM) BAB VI Simpulan dan Saran.pdf Download (244kB)
	Text (Karya Tulis Ilmiah 2026 D-III TLM) Daftar Pustaka.pdf Download (266kB)
	Text (Karya Tulis Ilmiah 2026 D-III TLM) Lampiran-Lampiran.pdf Restricted to Repository staff only Download (539kB) | Request a copy

Abstract

IDENTIFICATION OF METHICILLIN-RESISTANT Staphylococcus aureus (MRSA) ON THE HANDS OF SANITATION WORKERS AT TPS 3R KUBU LESTARI. Background: Waste generation in Pemogan Village reaches 51.8 tons per day, while TPS 3R Kubu Lestari only processes a maximum of 8 tons daily. This overload causes waste to accumulate and decompose uncontrollably, forming a moist, organic rich environment that supports the growth of pathogenic microbes, including Staphylococcus aureus. This condition increases the risk of bacterial colonization on workers, including the antibiotic‑resistant strain MRSA, which is difficult to treat and may transmit infections. Objective: To identify MRSA on the hands of sanitation workers and measure its inhibition zone against cefoxitin. Methods: This descriptive study included 28 respondents selected by total sampling. Hand swabs were cultured on MSA medium, followed by catalase and coagulase tests, and antibiotic susceptibility testing using the disk diffusion method according to CLSI guidelines. Results: Of 28 samples, 8 (28.6%) tested positive for S. aureus, 3 isolates (37,5%) were MRSA (zone ≤21 mm), and 5 (62,5%) were MSSA (zone ≥22 mm). Conclusion: MRSA colonization was found in 37,5% of samples collected from sanitation workers at TPS 3R Kubu Lestari. IDENTIFIKASI METHICILLIN RESISTANT Staphylococcus aureus (MRSA) PADA TANGAN PETUGAS KEBERSIHAN DI TPS 3R KUBU LESTARI Latar Belakang: Volume timbulan sampah di Desa Pemogan mencapai 51,8 ton per hari, sedangkan TPS 3R Kubu Lestari hanya mampu mengolah maksimal 8 ton per hari. Kelebihan beban ini menyebabkan sampah menumpuk dan terurai tanpa pengendalian, membentuk lingkungan lembap serta kaya zat organik yang mendukung pertumbuhan mikroba patogen, termasuk Staphylococcus aureus. Kondisi ini berpeluang bagi petugas terdapat kolonisasi bakteri, termasuk jenis resistan antibiotik atau MRSA yang sulit diobati dan berisiko menularkan infeksi. Tujuan: Mengidentifikasi keberadaan MRSA pada tangan petugas kebersihan serta mengukur zona hambat pertumbuhan bakteri terhadap antibiotik cefoxitin. Metode: Penelitian bersifat deskriptif dengan melibatkan 28 responden secara total sampling. Sampel usapan tangan diuji pada media MSA, dilanjutkan uji biokimia katalase dan koagulase, serta uji kepekaan antibiotik metode difusi cakram sesuai standar Clinical and Laboratory Standards Institute (CLSI). Hasil: Dari 28 sampel, sebanyak 8 sampel (28,6%) positif mengandung S. aureus, terdiri dari 3 MRSA (37,5%, zona ≤21 mm) dan 5 MSSA (62,5%, zona ≥22 mm). Simpulan: Ditemukan kolonisasi MRSA pada tangan petugas kebersihan di TPS 3R Kubu Lestari sebesar 37,5% dari total sampel yang diperiksa.

Actions (login required)

View Item