PERBANDINGAN HASIL PEMERIKSAAN HEMOGLOBIN METODE MANUAL CYANMETHEMOGLOBIN DENGAN METODE OTOMATIS HEMATOLOGY ANALYZER

Dewi, Putu Shinta Letisia (2026) PERBANDINGAN HASIL PEMERIKSAAN HEMOGLOBIN METODE MANUAL CYANMETHEMOGLOBIN DENGAN METODE OTOMATIS HEMATOLOGY ANALYZER. Diploma thesis, Poltekkes Kemenkes Denpasar Jurusan Teknologi Laboratorium Medis 2026.

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Abstract

COMPARISON OF HEMOGLOBIN EXAMINATION RESULTS USING THE MANUAL CYANMETHEMOGLOBIN METHOD AND THE AUTOMATED HEMATOLOGY ANALYZER METHOD ABSTRACT Background : Hemoglobin is a protein in erythrocytes that plays an important role in transporting oxygen from the lungs to all body tissues. Hemoglobin level examination is one of the most frequently performed hematological tests to assist in diagnosing various conditions, particularly anemia. Hemoglobin measurement can be performed using the manual cyanmethemoglobin method or automated methods using a hematology analyzer. Differences in the working principles of these methods may lead to variations in examination results; therefore, research is needed to determine the differences in the results obtained. Purpose : This study aimed to determine the differences in hemoglobin examination results using the cyanmethemoglobin method and the hematology analyzer method. Method : This study used a comparative descriptive design with a quantitative approach. The samples consisted of 32 venous blood samples using EDTA anticoagulant. Hemoglobin levels were examined using the cyanmethemoglobin method and a hematology analyzer. Data analysis was performed using the Shapiro–Wilk normality test, Levene’s Test for homogeneity, and the Independent Sample T-Test. Result : The mean hemoglobin level using the cyanmethemoglobin method was 12.6 g/dL, while the hematology analyzer method showed a mean value of 13.2 g/dL. The Independent Sample T-Test showed a significance value of p = 0.020 (p < 0.05), indicating a significant difference between the two methods. Conclusion : There is a significant difference between hemoglobin measurement results using the cyanmethemoglobin method and the hematology analyzer method. Keywords : Hemoglobin, Cyanmethemoglobin, Hematology Analyzer. PERBANDINGAN HASIL PEMERIKSAAN HEMOGLOBIN METODE MANUAL CYANMETHEMOGLOBIN DENGAN METODE OTOMATIS HEMATOLOGY ANALYZER ABSTRAK Latar belakang : Hemoglobin merupakan protein dalam eritrosit yang berperan penting dalam proses transport oksigen dari paru-paru ke seluruh jaringan tubuh. Pemeriksaan kadar hemoglobin termasuk salah satu pemeriksaan hematologi yang sering dilakukan untuk membantu menegakkan diagnosis berbagai kondisi, terutama anemia. Penentuan kadar hemoglobin dapat dilakukan dengan metode manual cyanmethemoglobin maupun metode otomatis menggunakan hematology analyzer. Perbedaan prinsip kerja pada kedua metode tersebut memungkinkan terjadinya variasi hasil pemeriksaan sehingga perlu dilakukan penelitian untuk mengetahui perbedaan hasil yang dihasilkan. Tujuan : Penelitian ini bertujuan untuk mengetahui perbedaan hasil pemeriksaan kadar hemoglobin menggunakan metode cyanmethemoglobin dan hematology analyzer. Metode : Penelitian ini menggunakan desain deskriptif komparatif dengan pendekatan kuantitatif. Sampel penelitian terdiri dari 32 sampel darah vena yang menggunakan antikoagulan EDTA. Pemeriksaan kadar hemoglobin dilakukan dengan metode cyanmethemoglobin dan hematology analyzer. Analisis data dilakukan menggunakan uji normalitas Shapiro-Wilk, uji homogenitas Levene’s Test, serta uji Independent Sample T-Test. Hasil : Rata-rata kadar hemoglobin dengan metode cyanmethemoglobin adalah 12,6 g/dL, sedangkan metode hematology analyzer sebesar 13,2 g/dL. Hasil uji Independent Sample T-Test menunjukkan nilai signifikansi p=0,020 (p<0,05) yang menunjukkan adanya perbedaan bermakna antara kedua metode. Simpulan : Terdapat perbedaan signifikan antara hasil pemeriksaan kadar hemoglobin menggunakan metode cyanmethemoglobin dan hematology analyzer. Kata kunci : Hemoglobin, Cyanmethemoglobin, Hematology Analyzer.

Item Type: Thesis (Diploma)
Uncontrolled Keywords: Hemoglobin, Cyanmethemoglobin, Hematology Analyzer.
Subjects: L Education > L Education (General)
Q Science > Q Science (General)
Divisions: Jurusan Analis Kesehatan
Depositing User: Paramita Ida Ayu Nia
Date Deposited: 29 Oct 2026 03:17
Last Modified: 29 Oct 2026 03:17
URI: http://repository.poltekkes-denpasar.ac.id/id/eprint/20708

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