UJI DAYA HAMBAT EKSTRAK DAUN PEPAYA (Carica Papaya Linn) TERHADAP PERTUMBUHAN BAKTERI Shigella dysentriae

Praningtyas, Ni Kadek Yuni (2025) UJI DAYA HAMBAT EKSTRAK DAUN PEPAYA (Carica Papaya Linn) TERHADAP PERTUMBUHAN BAKTERI Shigella dysentriae. Diploma thesis, Poltekkes Kemenkes Denpasar Jurusan Teknologi Laboratorium Medis 2025.

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Abstract

INHIBITORY TEST OF PAPAYA LEAF EXTRACT (Carica Papaya Linn) AGAINST THE GROWTH OF Shigella dysentriae BACTERIA. Background: Shigella dysenteriae is a pathogenic bacterium that causes infection and has developed resistance to antibiotics. Papaya leaves contain flavonoids, alkaloids, saponins, and tannins, which act as antibacterial agents. Purpose: this study aimed to determine the inhibitory effect of papaya leaf extract on the growth of Shigella dysenteriae. Method: this research is an experimental study using a “Posttest Only Controlled Group Design” with the disk diffusion method at four concentrations: 20%, 40%, 60%, and 80%, chloramphenicol 30 µg was used as the positive control, and 96% ethanol was used as the negative control. Results: the results showed that papaya leaf extract inhibited Shigella dysenteriae, with inhibition zone diameters of 5,88 mm ± 0,10 (20%), 6,28 mm ± 0,10 (40%), 6,68 mm ± 0,10 (60%), and 7,00 mm ± 0,08 (80%). The LSD test showed a significance value of ρ˂α (0.05), indicating a difference in inhibition zones among the various concentrations of papaya leaf extract. Conclusion: of this study is that there are significant differences in the inhibition zones of Shigella dysenteriae growth based on the concentrations of papaya leaf extract. Keywords: Papaya leaf, Inhibition zone, Shigella dysenteriae. UJI DAYA HAMBAT EKSTRAK DAUN PEPAYA (Carica Papaya Linn) TERHADAP PERTUMBUHAN BAKTERI Shigella dysentriae. Latar Belakang: Shigella dysentriae merupakan bakteri patogen penyebab infeksi yang mengalami resistensi terhadap antibiotik. Daun pepaya mengandung senyawa flavonoid, alkaloid, saponin, tanin yang berperan sebagai zat antibakteri. Tujuan: penelitian ini bertujuan untuk mengetahui daya hambat ekstrak daun pepaya terhadap pertumbuhan bakteri Shigella dysentrie. Metode: penelitian ini merupakan eksperimen dengan rancangan “Posttest Only Controlled Group Design” menggunakan metode difusi cakram dengan empat konsentrasi yaitu 20, 40, 60, dan 80%, kontrol kerja menggunakan kloramfenikol 30 µg dan kontrol negatif menggunakan etanol 96%. Hasil: penelitian menunjukkan ekstrak daun pepaya mampu menghambat Shigella dysentriae dengan diameter zona hambat 20% sebesar 5,88 mm±0,10, 40% sebesar 6,28 mm±0,10, 60% sebesar 6,68 mm±0,10, 80% sebesar 7,00 mm±0,08. Uji LSD menunjukkan nilai ρ˂α (0,05) sehingga ada perbedaan zona hambat pertumbuhan Shigella dysentriae antara berbagai konsentrasi ekstrak daun pepaya. Simpulan: berdasarkan penelitian ini terdapat perbedaan zona hambat dari variasi ekstrak daun pepaya terhadap pertumbuhan bakteri Shigella dysentriae. Kata kunci: Daun pepaya, Zona hambat, Shigella dysentriae

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