HUBUNGAN PERSONAL HYGIENE PEDAGANG AYAM POTONG TERHADAP HASIL UJI TPC (TOTAL PLATE COUNT) DI DESA ABIANSEMAL

Sari, Ni Luh Putu Ayu Pramudita (2025) HUBUNGAN PERSONAL HYGIENE PEDAGANG AYAM POTONG TERHADAP HASIL UJI TPC (TOTAL PLATE COUNT) DI DESA ABIANSEMAL. Diploma thesis, Poltekkes Kemenkes Denpasar Jurusan Teknologi Laboratorium Medis 2024.

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Abstract

ABSTRACT Diarrhea is a health problem that often occurs in Indonesia. Based on the health profile of the Badung district health office, the coverage of finding diarrhea cases of all ages at the Abiansemal I Health Center in 2022 is 37.3%. Generally, the factors that affect the percentage of diarrhea cases in Abiansemal are contamination that causes diarrhea that enters the body through unhygienic food consumption. This study aims to determine whether there is a relationship between personal hygiene of slaughtered chicken traders to the results of the TPC (Total Plate Count) test in Abiansemal Village. This type of research is quantitative analytical survey method. With the subject of research 21 slaughtered chicken traders and 21 samples of cut chicken meat. Bacteriological quality testing of Total Plate Count using PCA media with pour plate method and multilevel dilution technique was carried out. Meanwhile, in measuring personal hygiene, traders are carried out by means of interviews guided by questionnaires. The results of this study from 21 samples of mostly slaughtered chicken traders with poor personal hygiene all did not meet the TPC test requirements (100%). Based on the chi square test, p value (0.026) < α (0.05) was obtained, thus showing that there is a relationship between personal hygiene of slaughtered chicken traders and the total plate count results of slaughtered chicken sold in Abiansemal Village. Keywords : Diarrhea, Chicken Meat, Personal Hygiene, Total Plate Count ABSTRAK Diare adalah masalah Kesehatan yang sering terjadi di Indonesia. Berdasarkan profil kesehatan dinas kesehatan kabupaten Badung, cakupan penemuan kasus diare semua umur pada Puskesmas Abiansemal I tahun 2022 sebesar 37,3%. Umumnya faktor-faktor yang berpengaruh pada presentase kasus diare di Abiansemal adalah adanya kontaminasi penyebab diare yang masuk kedalam tubuh melalui konsumsi pangan yang tidak higenis. Penelitian ini bertujuan untuk mengetahui apakah terdapat hubungan personal hygiene pedagang ayam potong terhadap hasil uji TPC (Total Plate Count) di Desa Abiansemal. Penelitian ini menggunakan pendekatan kuantitatif dengan metode survei analitik. Subjek penelitian melibatkan 21 pedagang ayam potong dan 21 sampel daging ayam potong. Uji kualitas bakteriologis dilakukan dengan mengukur TPC menggunakan media PCA dengan metode pour plate dan teknik pengenceran bertingkat. Sedangkan dalam mengukur personal hygiene pedagang dilakukan dengan cara wawancara berpedoman pada lembar kuisioner. Hasil penelitian ini dari 21 sampel sebagian besar pedagang ayam potong dengan personal hygiene tidak baik semuanya tdiak memenuhi syarat uji TPC (100%). Berdasarkan hasil uji chi square, diperoleh nilai p (0,026) < α (0,05), yang menunjukkan terdapat hubungan antara personal hygiene dari pedagang ayam potong dengan hasil total plate count pada ayam potong yang dijual di Desa Abiansemal. Kata kunci: Diare, Daging Ayam, Personal Hygiene, Total Plate Count

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